Cell Counting With out Slides to Scale back Waste and Price

insights from businessDaniel SchiefferExpertise DirectorDeNovix

An interview with Daniel Schieffer, Expertise Director, DeNovix

Why are cells presently counted with slides? What number of occasions are the slides sometimes used and what occurs to them after they’re used?

Till not too long ago, cells had been sometimes counted on a reusable quartz hemocytometer. With the appearance of automated cell counters 12-15 years in the past, disposable slides turned widespread. This was primarily a method for these cell counters to load and depend slides. Whereas they’re handy for automated techniques, they introduce a further ongoing value for labs, and the single-use plastic additionally has a big environmental footprint.

Cells

Picture credit score: Shutterstock | Watiporn Yenchum

Every slide is designed to solely be used as soon as. There is likely to be two counts per slide, however they’re all single-use and are routinely disposed of after use. We have heard tales of individuals making an attempt to reuse single-use slides; nevertheless, for the sake of accuracy this isn’t beneficial by slide producers. It additionally provides considerably to the hands-on time required to depend cells.

How does the CellDrop get rid of disposable slides? The place are the cells saved and the way is full cleansing achieved between runs?

To enhance this technique DeNovix mixed the perfect of the outdated and new, changing plastic slides with two everlasting, optical grade sapphire surfaces. The surfaces are positioned parallel to at least one one other by a excessive precision screw to type a chamber analogous to a standard hemocytometer. The core expertise is customized from our DS-11 Collection of microvolume spectrophotometers and customers are instantly acquainted with the load, measure and wipe clear workflow.

Get rid of Prices | Automate Cell Counting

Researchers dispense the cells into the chamber, press depend and the evaluation is carried out mechanically. Afterwards, the pattern is solely wiped away with a dry laboratory wipe and the CellDrop is prepared for the subsequent pattern. One of many nice options of the CellDrop is a big, high-definition display screen with a stay preview of the measurement chamber. This enables the person to immediately verify that the surfaces are clear.

Using CellDrop technology

Picture credit score: DeNovix

How does the efficiency examine to slide-based cell counters?

The slide-based counters use plastic slides that introduce inconsistencies between counts. For instance, if a researcher has 10 plastic slides, there might be 10 slight variations between every of the slides. The CellDrop system makes use of a strong sapphire floor that’s constant every time it’s used, and the chamber heights are mechanically calibrated each time the instrument is turned on.

It’s totally reproducible run to run. As well as, cell counts on CellDrop are very quick in comparison with different automated counters, so there’s an actual efficiency improve within the person expertise.

CellDrop

Picture credit score: DeNovix

What purposes does the CellDrop work with?

Our most important purposes are presently in cell viability, i.e. stay/useless counts for mammalian, yeast and bug cell tradition. There are two fashions of the CellDrop obtainable—twin fluorescence and brightfield, or brightfield solely.

For brightfield viability measurements, scientists use a dye comparable to Trypan blue to stain cells. Trypan blue has its limitations, so for labs requiring higher specificity and accuracy in addition to a higher vary of purposes, we advocate the usage of fluorescence. The CellDrop can use frequent fluorophores comparable to Acridine orange and Propidium iodide (AO/PI), the place the fluorescent AO stains stay cells inexperienced and PI stains useless cells crimson.

Utilizing this fully takes the guesswork out of counting stay/useless cells or counting main cells in samples which are probably contaminated. For instance, it avoids counting crimson blood cells or different bits and particles {that a} typical Trypan algorithm or handbook counting may confuse for a cell, which results in an inaccurate depend.

You may also analyze GFP tranfected cells with CellDrop, offering the % viability and transfection effectivity. The platform is designed to be open to a variety of purposes throughout cell biology but in addition environmental, meals and brewing industries.

Can the CellDrop be used with hazardous and infectious samples?

There are two methods to deal with hazardous or infectious samples with the CellDrop. First, we designed it to take a seat in a biosafety cupboard. Researchers with bio-safety degree 2 or bio-safety degree 3 lab can put their CellDrop straight into the hood the place they’re coping with hazardous cells.

Analysis continues as regular, utilizing a typical cleansing protocol on the CellDrop after the cell samples have been counted. For instance, if 10% bleach is used to sterilize the gear and space used, this may be sprayed on a paper towel to wipe down the CellDrop after use.

We additionally permit for the usage of disposable slides inside the CellDrop for the aim of infectious or hazardous materials. If there’s a pattern that for no matter motive a lab actually does not wish to contact the CellDrop straight, a spread of slides from totally different producers can be utilized.

On this case, the researcher would put the slide on high of the reusable floor after which concentrate on the cells on the slide, depend it, after which throw it into the hazardous waste with out the pattern ever having touched the CellDrop.

What software program and algorithms/AI include the CellDrop cell counter? How do these assist scientists?

DeNovix software program is designed by life scientists for all times scientists. We work exhausting to make the software program not solely intuitive to make use of, but in addition very highly effective. Researchers can shortly depend and analyze outcomes instantly on board the instrument. The counting purposes and all the embedded algorithms are preinstalled on CellDrop counters, so there isn’t any want for added tablets, computer systems or cloud-based software program.

Inside every counting software, we’ve included a default protocol that works properly for commonest cell traces. Prospects can simply optimize settings for various cell varieties after which save these in protocols for added counts. Outcomes are saved onboard for straightforward retrieval for re-analysis or audit functions.

What limitations are there for the CellDrop system?

The system was engineered as a cell counter. So like all optically designed instrument, there are decision constraints as a result of physics of the system. We will reliably depend cells down 4 microns and there actually aren’t any limitations in comparison with what you are able to do with a handbook depend or a unique automated cell counter.

We have designed it to offer the perfect of each worlds—the pace of automation however with out the extra value and inconvenience of disposable slides. In the event you want single-use disposable slides for any motive the CellDrop can accommodate that, however it additionally provides researchers the flexibleness to make use of a reusable system for almost all of analysis they run.

What does the longer term maintain for DeNovix Cell Counters?

We’ll proceed to develop the purposes and performance of the CellDrop with our free software program updates. We work intently with our prospects and have already got some nice concepts that we’re planning to implement.

Extra broadly, DeNovix goes to proceed to search for locations the place we are able to innovate to avoid wasting prospects’ money and time and cut back ache factors within the lab. We wish to simplify instrumentation and supply instruments scientists wish to have however that aren’t cost-effective given the present expertise or want to purchase into a brand new consumable stream.

The place can readers discover extra info?

https://www.denovix.com/celldrop/

About Daniel SchiefferDaniel Schieffer

Dan joined NanoDrop Applied sciences Inc. in 2006 whereas finishing his BS in Biotechnology at College of Delaware. After contributing to the event of the NanoDrop 8000 and NanoDrop 2000 Dan accepted a instructing and analysis assistantship at Villanova College.

At Villanova he helped develop a novel circulate cytometry-based FRET system to elucidate the function of cell membrane group in CD4+ T Cell activation.

Dan then moved right into a analysis assistantship function on the College of Pennsylvania the place he carried out excessive throughput genetic screens to find novel immune system pathways that regulate pathogenesis in insect born viruses earlier than becoming a member of DeNovix in 2013. He has an MS in Immunology from Villanova College.

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