The antimicrobial properties of tea tree and hemp seed oils

There have been elevated calls for by shoppers for pure compounds able to enhancing meals high quality and security. Consequently, many have proposed the substitution of artificial antioxidants with plant extracts.

Bioactive tea tree oil (TTO) and hemp seed oil (HSO) are of explicit curiosity attributable to their antioxidant and antimicrobial properties.

Study: The Biological Activity of Tea Tree Oil and Hemp Seed Oil. Image Credit: ronstik /

Research: The Biological Activity of Tea Tree Oil and Hemp Seed Oil. Picture Credit score: ronstik /


TTO is obtained from the Melaleuca alternifolia plant of the Myrtle household and is well-known for its antimicrobial properties.

A tea tree is a tree or shrub that constitutes a papery bark and may develop as much as 14 meters in peak. This plant originated from Australia and may thrive in principally heat temperatures. Steam distillation is usually used for the extraction of lively elements of the tea tree plant, most of that are terpenes.

Following harvest, chilly urgent of hemp seeds permits for the extraction of HSO. Cannabidiol (CBD) content material in HSO may be both low or excessive; nevertheless, no comparability relating to the bioactivity of HSOs with totally different CBD contents with that of TTO has been made.

Together with the antibacterial properties related to many plant oils, these extracts are additionally related to anti-fungal properties. In actual fact, some plant oils can resist the hostile impression of harsh chemical compounds used to sanitize food-processing areas.

This has led to an elevated demand for improved antimicrobials used within the meals business. Subsequently, discovering pure antimicrobials that aren’t related to poisonous results is essential.

Antioxidants are artificial or pure substances that help within the neutralization of dangerous reactive oxygen species (ROS). These brokers assist to keep away from oxidative stress that arises attributable to an imbalance between oxidants and antioxidants.

Oxidative stress may be attributable to exterior components corresponding to smoking, publicity to the solar with out safety, or alcohol consumption. Excessive ranges of ROS for lengthy intervals also can result in ageing and lots of persistent ailments. Two plant extracts which can be recognized for his or her antioxidant properties embrace sesame oil and vetiver oil.

A brand new Applied Microbiology research compares the antimicrobial exercise of HSO and TTO merchandise in opposition to pathogenic micro organism corresponding to Salmonella enteritidis (S. enteritidis), Escherichia coli (E. coli), and Staphylococcus aureus (S. aureus). The researchers additionally assessed the antioxidant properties of those brokers, in addition to the existence of any interrelationship between their antimicrobial and antioxidant properties.

Concerning the research

The present research utilized antimicrobial and antioxidant assays. The antimicrobial assays included time-kill research, minimal inhibition focus (MIC) analysis, and the Kirby–Bauer disk diffusion methodology for analysis of antimicrobial properties of the samples.

Comparatively, the antioxidant assay was used to find out the focus of antioxidants within the pattern. This assay was carried out utilizing the two,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical-scavenging exercise methodology.

The antimicrobial and antioxidant exercise of seven oils, together with tea tree, sesame, rosehip, vetiver, pure hemp, natural hemp, and 5% CBD oil, had been assessed.

The measurement of the inhibition zone of tea tree oil on different bacteria: (a) S. aureus and (b) E. coli.The measurement of the inhibition zone of tea tree oil on totally different micro organism: (a) S. aureus and (b) E. coli.

Research findings

The onset of micro organism was reported to be six hours, whereas the best focus was noticed at 24 hours. The variety of micro organism within the TTO group was decrease than within the HSO teams.

The MIC and disk diffusion methodology outcomes indicated that out of all oils, TTO exhibited important antimicrobial exercise in opposition to all three pathogens. The MIC vary for TTO was reported to be 8, 2, and eight mg/mL for S. aureusS. enteritidis, and E.coli, respectively. The inhibition zones had been reported to be 2.19, 1.87, and a couple of.02 cm in opposition to S. enteritidisS. aureus, and E.coli, respectively.

Conversely, 5% CBD oil was discovered to supply the best antioxidant exercise that was 5.7 instances stronger as in comparison with that of TTO. Pure hemp and natural hemp additionally confirmed promising antioxidant properties. 

​​​​​​​IC50 values of the antioxidant activities of the different oils.IC50 values of the antioxidant actions of the totally different oils.


The present research decided TTO to be the best antimicrobial agent in opposition to E.coliS. aureus, and S. enteritidis. Moreover, the CBD content material of HSOs was discovered to play an essential position of their antioxidant properties. Substances with greater CBD exhibited higher antioxidant properties.

Subsequently, each TTO and CBD crops may be helpful in meals science research to make meals merchandise secure for shoppers. Additional analysis is required to optimize the antimicrobial and antioxidant properties of those oils for his or her use within the meals and medical industries.

Journal reference:
  • Lakatos, M., Apori, S. O., Dunne, J., & Tian, F. (2022). The Organic Exercise of Tea Tree Oil and Hemp Seed Oil. Utilized Microbiology. doi:10.3390/applmicrobiol2030041.



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